Table 4.

Proteins involved in redox systems, photosystems, signalling, transport, protein synthesis, assembly and turnover, plastid division, or unknown functions that were detected and proposed to be wheat amyloplast constituents¹.

Redox systems
Ferredoxin-thioredoxin system
113 Ferredoxin III (no EC)	2(ch), 3(ch), 4
114 Ferredoxin-NADP+ reductase EC 1.18.1.2 – non-green isoform	2(ch, pl), 3(ch), 4
115 Ferredoxin-thioredoxin reductase, variable subunit EC not available	2(ch), 3(ch), 4
Free radical scavenger system
116 Ascorbate peroxidase EC 1.11.1.11	2(ch, cy), 3(ch), 4
117 Cu/Zn superoxide dismutase EC 1.15.1.1	2(ch, cy, mi), 3(ch), 4
118 Glutaredoxin EC 1.20.4.1	2(ch), 3(ch), 4
119 Monodehydroascorbate reductase EC 1.6.5.4	2(ch, cy, mi), 3(ch), 4
120 Peroxiredoxin EC 1.11.1.15	2(ch), 3(ot), 4
121 Peroxiredoxin (Type 2) EC 1.11.1.15	2(ch), 3(ch), 4
Photosystem I and II thylakoid proteins
122 ATP synthase alpha chain (encoded by chloroplast DNA) EC 3.6.3.14	2(ch), 3(ot), 4
123 Apocytochrome f precursor (encoded by chloroplast DNA) EC not available	2(ch), 3(ot) but known plastid protein)
124 Chlorophyll a/b binding protein of photosystem II EC not available	2(ch), 3(ch), 4
125 Chlorophyll a/b binding protein of photosystem II EC not available	2(ch), 3(ch), 4
126 23 kDa oxygen evolving protein of photosystem II EC not available	2(ch), 3(ch), 4
127 33 kDa oxygen evolving protein of photosystem II EC not available	2(ch), 3(ch), 4
128 Oxygen-evolving enhancer protein 2 EC not available	2(ch), 3(ch), 4
129 Photosystem I reaction center subunit II EC not available	2(ch), 3(ch), 4
Signaling
130 Inositol phosphate phosphatase EC 3.1.3.25	2(ch), 3(ch), 4
131 Inositol monophosphatase family protein	2(ch), 3(ch, mi) 4
Substrate transport
132 ABC-type transporter, ATPase component, EC 3.6.3.*	2(ch), 3(ch), 4
133 ABC-type transporter, putative EC 3.6.3.*	2(ch), 3(ch), 4
134 ADP-glucose transporter	2(ch, mi), 3(ch, ot) 4
135 Amino acid selective channel protein	2(ch),3(ot),4
Protein synthesis, import, assembly and turnover
Ribosomal Proteins
136 29 kDa ribonucleoprotein A	2(ch), 3(ch), 4
137 40S ribosomal protein S20	2(ch), 3(ch, ot), 4
138 Hypothetical protein OSJNBa0071K18.7 – unk, possible chloroplast ribosomal 52	2(ch), 3(ch, mi), 4
139 Cp31BHv (31 kDa ribonucleoprotein, chloroplast)	2(ch), 3(ch), 4
140 Ribosome recycling factor	2(ch), 3(ch), 4
141 Translational elongation factor Tu	2(ch), 3(ch), 4
142 Translational inhibitor protein, putative	2(ch), 3(ch), 4
Protein import
143 Translocon Tic40-like protein	2(ch), 3(ch), 4
144 Translocon Tic40	2(ch), 3(ot), 4
145 Translocon Tic110	2(ch), 3(ot), 4
146 Translocon Toc34-1	2(ch), 3(ot), 4
147 Translocon Toc75	2(ch), 3(ch), 4
148 Translocon Toc75	2(ch), 3(ch), 4
Assembly, folding, proteolysis and turnover
149 Aminopeptidase C, putative (Acyl-peptide hydrolase-like) EC 3.4.22.40	2(ch), 3(ch), 4
150 Chaperonin, 20 kDa, chloroplast	2(ch), 3(ch), 4
151 Chaperone GrpE	2(ch), 3(ch), 4
152 Clp C protease, ATP-binding subunit EC 3.4.21.92	2(ch, mi), 3(ch, mi), 4
153 Clp C protease, ATP-binding subunit EC 3.4.21.92
154 ClpB heat shock protein, putative	2(various), 3(ch)
155 Heat shock cognate 90 kDa protein, putative	2(various), 3(ch)
156 Oligopeptidase A-like EC 3.4.24.70 – mito?	2(ch), 3(ch), 4
157 Peptidyl-prolyl isomerase EC 5.2.1.8	2(unk), 3(mi), 4
158 Peptidyl-prolyl isomerase, Cyclophilin-like – mito?	2(ch), 3(ch), 4
159 Peptidyl-prolyl cis-trans isomerase-like protein	2(ch), 3(mi), 4
160 Plastid-lipid associated protein EC not available	2(ch), 3(ch), 4
161 Rubisco subunit binding-protein alpha subunit	2(ch), 3(ot, mi), 4
162 Rubisco subunit binding-protein beta subunit	2(ch), 3(ch), 4
163 Zinc metalloprotease EC 3.4.21.74	2(ch, mi), 3(ot), 4
Plastid division
164 FtsZ protein	2(ch), 3(ch), 4
165 Plastid division protein [Arc6]	2(ch), 3(ch), 4
Function is unknown or not verified, but probably located in plastid
166 Coenzyme F420 hydrogenase-like EC 1.12.99.1	2(pl), 3(ch)
167 Cytochrome b5 domain containing protein	2(ch), 3(ch)
168 Isoflavone reductase related protein EC 1.3.1.45 related	2(ch), 3(ch)
169 Hypothetical protein At1g26160 – putative metal-dependent phosphohydrolase; biological function unknown	2(pl), 3(ch)
170 Hypothetical protein At2g43940	2(ch), 3(ch)
171 Hypothetical protein At5g08540	2(pl), 3(ch)
172 Hypothetical protein F24D7.19	2(ch), 3(ch, mi)
173 Hypothetical protein OSJNBa0038O10.22	2(ch), 3(ch)
174 Hypothetical protein OSJNBa0054L14.15, DUF573 family protein of unknown function	2(unk),3(ch)
175 Hypothetical protein OSJNBa0095E20.4 – CBS domain containing protein; predicted transcriptional regulator. CBS domains are found in otherwise unrelated proteins and enzymes however	2(unk), 3(ch)
176 Hypothetical protein Os06g0217700 – function unknown	2(unk), 3(ch)
177 Hypothetical protein Os08g0254900 – Fiber protein FB4	2(ch), 3(ch)
178 Hypothetical protein Os08g0254900 – Fiber protein FB4 same gene as above but different peptides and different 2DE spot	2(ch), 3(ch)
Function is unknown or not verified, but may be located in plastid and/or mitochondrion
180 Hypothetical protein At3g55760	3(ch, mi)
181 Hypothetical protein P0450E05.20	3(ch, mi)
182 Glycine-rich RNA-binding protein 2, putative	2(mi), 3(ch)
Probably non-plastid but mentioned in paper
183 Phosphoglucomutase EC 5.4.2.2	2(ch, cy), 3(ot),
184 Phosphoglycerate mutase EC 5.4.2.1	2(pl, cy), 3(ot)
185 Lactoylglutathione lyase EC 4.4.1.5	2(cy, mi), 3(ot)
186 6-phosphogluconate dehydrogenase EC 1.1.1.44	2(am, ch, cy, pl), 3(sp)

¹Criteria: as in Table 1.