Table 2.
Mutations found in isolated strains with enhanced survival upon leucine starvation
| Gene | Mutation (chr:bp) | Protein | Parent | Strain | Other mutations in |
|---|---|---|---|---|---|
| PPM1 | 4:1333843 A to Δ | L49X | 1 | DBY11202 | CPR3, GCD1, ACF4, KEX1 |
| PPM1 | 4:1333461 71bpΔ | L146X | 1 | DBY11203 | |
| TOR1 | 10:559755 C to Δ | S116X | 1 | DBY11204 | |
| TOR1 | 10:563920* | S1509X | 1 | DBY11205 | |
| TOR1 | 10:565483 C to G | Y2025X | 1 | DBY11206 | ADP1 |
| SCH9 | 8:511331 G to A | G657S | 2 | DBY11211 | SSK22 |
| SCH9 | 8:509689 C to AA | N125X | 2 | DBY11214 | |
| HRD1 | 15:302637 C to T | Q134X | 1 | DBY11207 | |
| HRD1 | 15:301850 G to T | C396F | 1 | DBY11208 | CDC60 |
| HAP1 | 12:646559 C to G | S48X | 2 | DBY11213 | AUS1 |
| HAP1 | 12:647879 T to G | F488C | 2 | DBY11215 | |
| KOG1 | 8:480255 T to C | S140 P | 2 | DBY11212 | |
| TPD3 | 1:126722 G to C | D615H | 1 | DBY11210 | FLO1, FLO5 |
| HRD3 | 12:557609 G to T | G274C | 1 | DBY11210 | FLO1, FLO5 |
| 1 | DBY11209 | UBR2, 15:1042k to end |
Genes contributing to the gene neighborhood (Fig. 6) are given in the first column. Parent 1, FY strain DBY8863, parent 2, SK1 strain NKY1060. These 14 strains were randomly chosen from 27 independent selections.
*TTACGA to CTTCTTA