Table 1.
Comparison of 16S rRNA diversity recovered before and after capture of ANME-2c consortia, using Magneto-FISH
| 16S rRNA phylotypes, % |
Closest relative | |
|---|---|---|
| Bulk sediment clone library | Bead capture clone library | |
| Archaea | ||
| 26 | 92 | ANME-2c |
| 6 | 7 | ANME-2a |
| 2 | 1 | Methanococcoides burtonii |
| 19 | 0 | ANME-2b |
| 44 | 0 | Others (ANME-1, MBGD, MBGB) |
| Bacteria | ||
| 25 | 42 | Deltaproteobacteria (Desulfobulbus or Desulfosarcina) |
| 0 | 32 | Betaproteobacteria (Burkholderiaceae) |
| 0 | 9 | Alphaproteobacteria (Sphingomonas) |
| 9 | 2 | Gammaproteobacteria (uncultured) |
| 16 | 7 | Epsilonproteobacteria (uncultured) |
| 0 | 4 | CFB group* (AJ535255, AY768987) |
| 0 | 3 | Planctomyces (AF424488, EF424502) |
| 50 | <1 | Others (eight additional major lineages) |
Archaeal and bacterial 16S rRNA clone libraries were constructed by using Ar20F-958r and Ba27F-1492r, respectively. Total number of clones in archaeal library: 211 (bead capture) and 254 (bulk sediment). Total number of clones in bacterial library: 139 (bead capture) and 165 (bulk sediment).
*CFB group: Cytophaga/Flexibacter/Bacterioides.