Fig. 2.

Altered positioning and numbers of immature neurons. (A and B) Distribution of immature neurons in the GCL. (A) Representative images of DCX-positive neurons from HOM mutant Disc1 mice and their WT littermates. Note the presence of DCX-positive neurons in the deeper layer of the GCL (arrows) of mutant mice. The dotted line represents the outer edge of the GCL. (B) Quantification of neuronal distribution performed by using the χ² test in a contingency analysis. n = 54 cells were analyzed for each genotype. (C–E) Dendritic orientation of immature neurons. (C) Representative images of DCX-positive neurons from HOM mutant Disc1 mice and their WT littermates. (D and E) Quantification was performed by defining the angle of orientation (θ) for each apical dendrite (D) and calculating the mean change in θ (E). (F and G) Quantification of proliferating cells in the CGL. (F) Representative images of DCX-positive immature neurons (Upper) and BrdU-labeled neural precursor cells (Lower) in the DG of HOM mutant Disc1 mice as compared with their WT littermates. (G) Quantification of DCX-positive immature neurons (Left) and BrdU-labeled neural precursor cells (Right). Values represent mean ± SEM. ∗, P < 0.05; ∗∗∗, P < 0.0001. (Scale bars: A and F, 100 μm; C, 25 μm.)