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Cooperation between Osx and Dkk1 in the inhibition of Wnt3A-induced Topflash activity. Topflash and Fopflash reporters were transfected in HEK293 cells with pEX-Osx as indicated. Wnt3A-conditioned medium was used to activate the Topflash reporter. Recombinant Dkk1 protein was added to the medium at the different concentrations as indicated. Results were expressed as the ratio of Topflash over Fopflash activity.
