Fig. 4.

Mutations in candidate innate immune regulators affect expression of many candidate antimicrobial genes. RNA was prepared from the indicated nematode strains and expression of the indicated antimicrobial genes was measured by using qPCR. Gene expression for each mutant is compared to the wild-type strain. Expression values that were significantly different from wild type (P < 0.05, t test) are indicated with an asterisk. Mutant alleles used are: acy-1(md1756), tub-2(gk417), tbc-1(tm2282), and vab-10(e698).