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Epigenetic alterations silence miRNA expression in ovarian cancer. (A) Heat map depicts expression of 24 miRNAs in IOSE cells and EOC cell lines analyzed by real-time RT-PCR. (B) Expression of the same 24 miRNAs in five EOC cell lines and HeLa cells after treatment with demethylating agent 5-aza-2′-deoxycytidine (5-Aza-CdR) and the histone deacetylase inhibitor 4-phenylbutyric acid (PBA) for 6 days.
