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. 2008 May 28;3(5):e2315. doi: 10.1371/journal.pone.0002315

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Bhattacharya et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) DND1 interacts with endogenous APOBEC3. Mouse testis lysates were incubated with GST-DND1α (lane 1), GST-DND1β⪽ (lane 2) or GST protein (lane 3) and incubated with Sepharose 4B beads. The beads were washed and the contents eluted into loading dye prior to electrophoresis and immunoblotting with rabbit anti-mouse APOBEC3 antibody. Control lanes on the left are cell lysates from untransfected cells (lane 4) and lysates from cells transfected with myc-APOBEC3 expression constructs (lane 5). (b) Dnd1 and Apobec3 are expressed in embryonic germ (EG) cells and genital ridges. RT-PCR for Apobec3, Dnd1 and HPRT (hypoxanthine-guanine phosphoribosyltransferase) on total RNA from EG cells, mouse embryo fibroblasts (MEF), and E13.5 genital ridges (GR). (c) EG cells in culture, stained with alkaline phosphatase (orange clusters), growing on MEF cell layer.