Table 1. Amount of an 80bp mitochondrial fragment (number of templates per real-time PCR reaction, with 5 µl DNA extracts used in 25 µl reactions, average from two real-time amplifications), and allele status in seven samples of ancient human remains typed in three laboratories (Linköping, Stockholm, and Uppsala).
Sample | No. of molecules | Allele distribution for the LCT gene at Stockholm/Uppsala | Allele distribution for the LCT gene at Linköping |
Ajv5 | 1263 | C | C |
Ajv14 | 2659 | C | C/T |
Ajv54 | 8714 | C/T | C |
Gök1 | 1395 | C | T |
Gök2 | 8988 | C | C |
Gök6 | 1389 | C | C/T |
Gök7 | 3249 | C/T | C/T |
T Freq | 0.14 | 0.36 |
The allele status in the Linköping samples was based on between 3 and 7 amplifications deriving from two independent extractions, while 3 amplifications deriving from one extraction in each case were made in Stockholm and one in Uppsala.