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. 2008 Apr;152(1):81–87. doi: 10.1111/j.1365-2249.2008.03605.x

Fig. 1.

Fig. 1

Purification of recombinant early secreted antigen target 6 (rESAT6) protein as determined by sodium dodecyl sulphate-polyacrylamide gel elecrtophoresis electrophoresis. Lane 1, molecular weight protein standards (Sigma). Lane 2, the purified rESAT6 protein. Lane 3, Escherichia coli extracts before isopropyl-b-D-thiogalactopyranoside induction. Lane 4, E. coli extracts 4 h after induction. The gel was subjected to electrophoresis followed by Coomassie blue staining.