Fig. 4.
(a) Effect of diphenyleneiodium (DPI) treatment on the reactive oxygen species (ROS) generation by thyrocytes of non-obese diabetic (NOD).H2h4 mice. A remarkable decrease in the intracellular ROS production was noted after treatment with DPI, a pharmacological inhibitor of enzymes that generate ROS. The solid histogram represents the intracellular ROS in response to sodium iodide (NaI) alone, and the open histogram shows the decreased fluorescence intensity (330 versus 220 respectively) after DPI treatment. The broken line histogram represents control (unstimulated and untreated) cells. (b) Effect of DPI treatment on adhesion molecule-1 (ICAM-1) expression on NOD.H2h4 mouse thyrocytes. ICAM-1 expression decreased after treatment with DPI. The solid histogram represents ICAM-1 in response to NaI alone, while the open histogram shows the decreased fluorescence intensity (199 versus 77 respectively) after DPI treatment. The broken line histogram represents control (unstimulated and untreated) cells.