Fig. 7.

Role plasminogen activators (PAs) on lymphocytes and hepatic stellate cell interaction: to isolate uPA's effect on lymphocytes and their role in fibrogenesis, wild-type (WT) mice were irradiated sublethally and then reconstituted with WT or urokinase-type plasminogen activator (uPA)^−/− lymphocytes. Next, fibrosis was induced by carbon tetrachloride; decreased T cells (a) and fibrosis (b) were identified in the uPA^−/− recipients. (a) Displays fluorescence activated cell sorter analysis of liver lymphocytes. Western blotting of beta actin and alpha smooth muscle actin in the liver protein extracts are presented in (b). Bands were scanned and quantified as described in Methods. Results were expressed as fold increase compared with naive mice from each gel and (b, right panel). Similar results were replicated in two different experiments.