Fig. 1.

Flow cytometric profiles of freshly isolated and cultured bone marrow stromal cells (BMSCs). (a) Non-haemopoietic mesenchymal stem cell-enriched cells, defined as CD45^–/CD106⁺ cells, were sorted immediately (gate 1) from the recipient of intrabone marrow-bone marrow transplantation (IBM-BMT) + IBM-donor lymphocyte infusion (DLI) after the staining of cells with fluorescein isothiocyanate (FITC)-anti-CD45 and phycoerythrin (PE)-anti-106 monoclonal antibodies (mAbs). The dot-plot profile of CD45^–/CD106⁺ cells from the recipients of IBM-BMT + intravenous (i.v.)-DLI or IBM-BMT alone (without DLI) was similar to (a). Haemopoietic bone marrow cell-enriched populations, sorted as CD45⁺/CD106^– cells (gate 2), were also prepared from the recipients, and used as controls. (b) Cultured BMSCs (for 2 weeks) obtained originally from the right tibia of the recipients of IBM-BMT + IBM-DLI were stained with FITC-anti-CD45 and PE-anti-106 mAbs, and analysed by a fluoresence activated cell sorter scan. The dot-plot profile of cultured BMSCs from the recipients of IBM-BMT + i.v.-DLI or IBM-BMT alone (without DLI) was similar to (b).