Fig. 3.

Inhibitory effect of cultured bone marrow stromal cells (BMSCs) on T cell proliferation. Bone marrow cells from the right tibia were collected from the recipients of intrabone marrow-bone marrow transplantation (IBM-BMT) + IBM-donor lymphocyte infusion (DLI), IBM-BMT + intravenous (i.v.)-DLI or IBM-BMT alone (without DLI) and cultured for 2 weeks. Graded numbers of cultured BMSCs (10²−10⁴ cells) were added to the culture of one-way mixed leucocyte reaction (MLR) (a, b). BMSCs were obtained after the long-term culture (cultured for 3 months), and were added to the culture of one-way MLR (c). The data in figures are expressed as mean counts per minute ± standard deviation of three mice (separately cultured BMSCs obtained from the recipient). Symbols in the boxes represent origins of cultured BMSCs. *Statistically significant when compared with MLRs performed in the groups (P < 0·05).