Fig. 5.

Production of transforming growth factor (TGF)-β and hepatocyte growth factor (HGF) in bone marrow stromal cells (BMSCs). Culture expanded BMSCs from the recipients of intrabone marrow-bone marrow transplantation (IBM-BMT) + IBM-donor lymphocyte infusion (DLI), IBM-BMT + intravenous (i.v.)-DLI or IBM-BMT alone (without DLI) were used for analysis of cytokine messages by real-time PCR. After DNase I treatment, cDNA was synthesized, amplified using HGF or TGF-β primer, and visualized with SYBR Green by real-time reverse transcription–polymerase chain reaction. Relative intensity of HGF or TGF-β mRNA was calculated on the basis of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) intensity. Columns represent relative cytokine message levels of TGF-β and HGF. Each column shows mean ± standard deviation of three mice (separately cultured BMSCs obtained from the recipient), and we performed two separate experiments. Symbols in the boxes represent origins of cultured BMSCs. *Statistically significant when compared with cytokine message performed in the groups (P < 0·05).