Table 1.
No DLI† | i.v.-DLI | IBM-DLI | T cells with ConA‡ | |
---|---|---|---|---|
IL-2 (pg/ml) | 0 | 0 | 0 | 87·3 ± 15·5 |
IFN-γ (pg/ml) | 0 | 0 | 0 | 1418·2 ± 369·4 |
IL-10 (pg/ml) | 0 | 0 | 0 | 1114·6 ± 103·1 |
TGF-β (ng/ml) | 0 | 0·27 ± 0·4 | 14·2 ± 2·4 | 0·6 ± 0·5 |
Bone marrow stromal cell (BMSC) culture supernatants from the recipients of intrabone marrow-bone marrow transplantation (IBM-BMT) + IBM-donor lymphocyte infusion (DLI), IBM-BMT + intravenous (i.v.)-DLI, or IBM-BMT alone (without DLI) were collected 2 weeks later. The cell supernatants were analysed for the amount of interleukin (IL)-2, IL-4, interferon (IFN)-γ and trandforming growth factor (TGF)-β by enzyme-linked immunosorbent assay.
Splenic T cells from BALB/c mice were activated with concanavalin A (ConA) and used as a positive control.