Table 2.

Analyses of cytokine messages by real-time reverse transcription–polymerase chain reaction (RT–PCR).

Cytokines examined	No DLI†	i.v.-DLI	IBM-DLI	T cells with ConA‡
IL-2	0·47 ± 0·3§	0·31 ± 0·2	0·47 ± 0·3	8·51 ± 6·1
IL-4	0	0	0·025 ± 0·03	1277·2 ± 357·4
IL-10	0	0	2·7 ± 2·3	95 000 ± 16 000
IL-15	0	0	0	n.d.

^†Culture expanded bone marrow stromal cells (BMSCs) from the recipients of intrabone marrow-bone marrow transplantation (IBM-BMT) + IBM-donor lymphocyte infusion (DLI), IBM-BMT + intravenous (i.v.)-DLI, or IBM-BMT alone (without DLI) were used for analysis of cytokine messages by real-time RT–PCR. After DNase I treatment, cDNA was synthesized, amplified using interleukin (IL)-2, IL-4, IL-10 or IL-15 primer, and visualized with SYBR Green by real-time RT–PCR.

^‡Splenic T cells from BALB/c mice were activated with concanavalin A (ConA) and used as a positive control.

^§Relative intensities of soluble factors were calculated on the basis of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA. Numbers in the table represent mean intensities of cytokines ± standard deviation of three mice (separately cultured BMSCs obtained from the recipient). We performed two separate experiments. n.d., not done.