Table 3.
Recombinant frequency and hot spot activity for heptamers created within the ura4 gene
| Heptamer allele | Test allele | Recombinant frequency (Ura+ per 106 spores) | Hot spot activity* |
|---|---|---|---|
| Heptamer in ura4 | |||
| ura4-167 (heptamer) | ura4-294 | 965, 768, 488, 679, 700 | 8.1 [3.4–17.5 (5)] |
| ura4-168 (control) | ura4-294 | 55, 87, 85, 202, 141 | NA |
| ura4-167 (heptamer) | ura4-595 | 1017, 703, 615, 1469, 927, 633 | 9.5 [4.7–19.8 (6)] |
| ura4-168 (control) | ura4-595 | 182, 121, 31, 160, 196, 54 | NA |
| Inversion in ura4 | |||
| ura4-169 (inversion) | ura4-294 | 138, 156, 190, 140, 136 | 4.8 [2.6–8.0 (5)] |
| ura4-170 (control) | ura4-294 | 54, 31, 24, 46, 25 | NA |
| ura4-169 (inversion) | ura4-595 | 227, 205, 237, 142, 159 | 5.3 [2.9–10.7 (5)] |
| ura4-170 (control) | ura4-595 | 78, 60, 22, 28, 35 | NA |
*
Hot spot activity is the recombinant frequency for the cross with the heptamer divided by that for the corresponding control. Crosses were performed in pairs, in the order indicated. Mean values are given; the range and number of independent crosses (n) performed are given in brackets. NA, not applicable.