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Serum levels of IgG1, IgG2a and total IgG in Rag2–/– mice that received unsorted wild-type spleen cells or CD25-depleted cells and showed a survival > 48 days or < 48 days. Serum was collected on the day the mice were killed and measurements were performed by enzyme-linked immunosorbent assay. Significance levels indicated for Mann–Whitney two-tailed test. Ig, immunoglobulin.
