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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: J Insect Physiol. 2008 Feb 7;54(4):708–718. doi: 10.1016/j.jinsphys.2008.01.011

Figure 1.

Figure 1

The effect of rapid cold hardening on the acute and chronic cold tolerance in four larval stages (instars I, II and III and wandering 3rd instars - w) of four strains of Drosophila melanogaster. Black bars are control animals, grey bars represent animals pre-treated at 0 °C for 1 hour. Acute exposures were at the previously-determined LT90 at −7 °C; chronic exposures were at the LT90 at −2 °C (see Tables 1 and 2 for details). Mean survival (± s.d.) was derived from 3 to 5 replicates each consisting of 10 − 20 larvae. Asterisks indicate significant differences between control and pre-treated animals: *p<0.05; **p<0.01; ***p<0.001.