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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: J Insect Physiol. 2008 Feb 7;54(4):708–718. doi: 10.1016/j.jinsphys.2008.01.011

Figure 2.

Figure 2

The effect of heat treatment on the acute and chronic cold tolerance in four larval stages (instars I, II, III and wandering late 3rd instars - w) of four strains of Drosophila melanogaster. Black bars represent control animals and grey bars represent animals pre-treated at 36.5 °C for 1 hour and recuperated for 1 more hour at 21 °C. Acute exposures were at the previously-determined LT90 at −7 °C and chronic exposures were at the LT90 for −2 °C (see Tables 1 & 2 for further details). Mean survival (± s.d.) was derived from 3 to 5 replicates each consisting of 10 − 20 larvae. Asterisks represents significant differences between the control and pre-treated animals: *p<0.05; **p<0.01; ***p<0.001.