Table 2.
Mean (± s.e.m.) time required for 10, 50 and 90 % mortality (LT10, LT50 and LT90, respectively) at −7 °C for four larval stages (instars I, II and II and wandering 3rd instars, W) of four wild-type strains of D. melanogaster. Values were derived from a generalized linear model of survival. Comparisons of models between stages within a strain and between strains within a stage were via a post hoc analysis (general linear hypothesis testing — glht in R; see methods for further details). Asterisks indicate significant differences between stages within strains and between strains within stages:
Strain/Stage | LT10 (minutes) | LT50 (minutes) | LT90 (minutes) | Nonlinear comparison between stages | Nonlinear comparison between strains | |
---|---|---|---|---|---|---|
Oregon R | I | 11.12±6.23 | 42.22±10.3 | 63.66±21.45 | II*** III** | be*** wn*** |
II | 25.1±7.85 | 49.27±7.85 | 73.3±21.30 | I*** | be*** wn** | |
III | <10 | 39.04±12.45 | 74.6±33.00 | I** | be** wn*** | |
W | <10 | 52.53±11.97 | 96.1±35.52 | be*** wn** | ||
Berlin K | I | 27.52±8.93 | 57.13±15.82 | 124.7±31.98 | or*** ca* wn*** | |
II | 29.6±11.01 | 76.12±15.37 | 122.62±42.0 | or*** ca* wn** | ||
III | 27.7±4.88 | 77.28±16.04 | 126.8±49.31 | or** ca*** wn*** | ||
W | 46.23±7.98 | 90.54±15.38 | 135.56±56.2 | or*** ca** wn** | ||
Canton S | I | <10 | 28.29±13.23 | 103.8±38.77 | II*** III** W ** | be* wn*** |
II | 13.1±6.2 | 48.5±21.88 | 68.27±24.5 | I** | be* wn** | |
III | <10 | 26.56±7.41 | 53.6±12.43 | I** W ** | be*** wn** | |
W | 22.32±7.811 | 51.5±24.24 | 83.83±20.4 | I** III** | be** wn** | |
W5N | I | 45.82±12.33 | 66.58±17.06 | 100.22±38.6 | or*** be*** ca*** | |
II | 47.43±10.96 | 71.5±31.62 | 96.27±32.01 | III** W** | or** be** ca** | |
III | 45.11±18.2 | 65.36±17.83 | 81.68±19.4 | II** | or*** be*** ca** | |
W | 28.8±9.02 | 52.81±35.72 | 99.33±41.54 | II** | or** be** ca** |
p < 0.05
p < 0.01
p < 0.001.