Fig. 4. TSG-6 enhances fibronectin binding to thrombospondin-1.
Uncoated wells (Δ) or wells coated using 10 µg/ml thrombospondin-1 (TSP1) were blocked with BSA and incubated with 0.5 µg/ml 125I-FN alone (▲) or in the presence of the indicated concentrations of full length TSG-6Q (●), CUB_C (▼), or Link_TSG6 (○). Results are presented as a percent of the input FN bound for quadruplicate determinations ± SD.