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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Matrix Biol. 2007 Oct 25;27(3):201–210. doi: 10.1016/j.matbio.2007.10.003

Fig. 5. TSG-6 does not inhibit integrin-mediated cell adhesion on fibronectin.

Fig. 5

A, Jurkat T cell adhesion on immobilized FN (5 µg/ml) was quantified in the presence of the indicated concentrations of TSG-6Q, Link_TSG6 or CUB_C after 20 min. incubation by colorimetric detection of cell-associated hexosaminidase activity. A significant increase relative to the FN control is indicated by * with p<0.05 by Student’s t-test. B, α5β1-mediated Jurkat T cell adhesion on immobilized recombinant r33 fragment of FN (10 µg/ml, 300 nM), which contains the RGD sequence recognized byα5β1 integrin, was determined by colorimetric detection of hexosaminidase activity and is presented as mean ± SD. Where indicated, the immobilized r33 FN was preincubated with the indicated concentrations of TSG-6Q or with Link_TSG6 for 30 min at 37 °C and then washed to remove unbound TSG-6 prior to adding Jurkat cells (solid bars) or used without washing (open bars).