Graphs. Upper: Dogs were bilaterally injected intrace-rebrally with 8.19 × 108 pfu of RAdLuc, plasmid DNA/PEI complexes (50 μg; N/P ratio = 6), or naked DNA (50 μg). One vector-treated cerebral hemisphere obtained in each dog was sliced into three sections (dorsal, medial, and ventral) of equal size, homogenized, and assayed for luciferase activity. Lower: Note the luciferase activity of pooled lysates from the entire cerebral hemisphere (normalized to expression in adenovirally injected brain). RLU = relative light unit; s = second.