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. Author manuscript; available in PMC: 2009 Mar 14.
Published in final edited form as: J Biol Chem. 2008 Jan 9;283(11):6668–6676. doi: 10.1074/jbc.M708372200

FIGURE 3. Dissociation of hemin from IsdA and IsdC.

FIGURE 3

HoloIsdA or holoIsdC at 3 μM was incubated with 48 μM H64Y/V68F apomyoglobin in 20 mM Tris-HCl, pH 8.0, at 25°C. A and B, spectra of the mixture at 0 h and 6 or 12 h after mixing. C, time courses of Δ(A600-A544) in these reactions of hemin dissociation. The grey lines are the theoretical curves obtained by fitting the data to a single exponential equation.