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. Author manuscript; available in PMC: 2009 Mar 14.
Published in final edited form as: J Biol Chem. 2008 Jan 9;283(11):6668–6676. doi: 10.1074/jbc.M708372200

FIGURE 4. Efficient hemin transfer from holoIsdA to apoIsdC.

FIGURE 4

ApoIsdC (47 μM) was incubated with 36 μM holoIsdA in 0.1 ml of 20 mM Tris-HCl, pH 8.0, at room temperature for 2 min. The two proteins were separated as described in the text. A, SDS-PAGE analysis showing separation of IsdA and IsdC from their mixture. Lanes: 1, holoIsdA/apoIsdC mixture before separation; 2, IsdA isolated from the mixture; 3, IsdC isolated from the mixture. B, spectra of holoIsdA (dashed curve) and treated IsdA (solid curve). C, spectra of apoIsdC (dashed curve) and treated IsdC (solid curve).