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HoloIsdA (33 μM) was incubated with 12, 24, 48, 71, or 95 μM apoIsdC in 20 mM Tris-HCl, pH 8.0, at 22°C for 20 min. Shown are the concentrations of holoIsdA, apoIsdA, and holoIsdC in the mixtures as a function of the ratio of initial [apoIsdC]/[holoIsdA].
