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(A) Structure of the tubulin/arylsulfatase chimeric gene, harbored in plasmid 1, containing the Volvox β-tubulin promoter region and the Volvox arylsulfatase coding region. (B) Plasmids 2 and 3 carry parts of the original plasmid 1. Plasmids 2 and 3 share a 776-bp overlap of arylsulfatase genomic sequence. B, BamHI; C, ClaI; E, EcoRI; K, KpnI; S, SalI.
