Abstract
Double-vial radiorespirometry was used to estimate the biodegradation rates of 14C-labeled phenol in a landfill leachate and a secondary treated domestic wastewater. Rates were found to be comparable for each material at each of the three concentrations tested. Sewage microorganisms immediately began biodegrading the [14C]phenol; landfill leachate microorganisms required a lag period before maximum biodegradation of the [14C]phenol. The apparent rate of [14C]phenol biodegradation was 2.4 times faster in the sewage than in the landfill leachate. Double-vial radiorespirometry was shown to be an effective method for screening biodegradation rates in aquifers.
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- Buddemeyer E. U. Liquid scintillation vial for cumulative and continuous radiometric measurement of in vitro metabolism. Appl Microbiol. 1974 Aug;28(2):177–180. doi: 10.1128/am.28.2.177-180.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coveney M. F., Wetzel R. G. Improved double-vial radiorespirometric technique for mineralization of C-labeled substrates. Appl Environ Microbiol. 1984 May;47(5):1154–1157. doi: 10.1128/aem.47.5.1154-1157.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FINA L. R., FISKIN A. M. The anaerobic decomposition of benzoic acid during methane fermentation. II. Fate of carbons one and seven. Arch Biochem Biophys. 1960 Dec;91:163–165. doi: 10.1016/0003-9861(60)90483-5. [DOI] [PubMed] [Google Scholar]
- King G. M., Wiebe W. J. Tracer analysis of methanogenesis in salt marsh soils. Appl Environ Microbiol. 1980 Apr;39(4):877–881. doi: 10.1128/aem.39.4.877-881.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McKinley V. L., Federle T. W., Vestal J. R. Improvements in and environmental applications of double-vial radiorespirometry for the study of microbial mineralization. Appl Environ Microbiol. 1983 Jan;45(1):255–259. doi: 10.1128/aem.45.1.255-259.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubin H. E., Subba-Rao R. V., Alexander M. Rates of mineralization of trace concentrations of aromatic compounds in lake water and sewage samples. Appl Environ Microbiol. 1982 May;43(5):1133–1138. doi: 10.1128/aem.43.5.1133-1138.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Subba-Rao R. V., Rubin H. E., Alexander M. Kinetics and extent of mineralization of organic chemicals at trace levels in freshwater and sewage. Appl Environ Microbiol. 1982 May;43(5):1139–1150. doi: 10.1128/aem.43.5.1139-1150.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]