Figure 4.

Rescue of SOD1 defect and increase of cellular copper concentration in ctr1 yeast by hCTR1 expression. (A) SOD1 activity is observed in hCTR1 expression strain. The control strains with vector alone or antisense hCTR1 have no detectable SOD1 activity. hCTR1-UTR(R), pDB20, and hCTR1-UTR(F) represent transformed ctr1 yeast strains with antisense UTR-less hCTR1, vector alone, and sense UTR-less hCTR1, respectively. The arrowhead indicates the SOD1 activity band. (B) hCTR1 expression in the ctr1 strain increases its cellular copper concentration. The y axis denotes the copper amount per milligram of protein. The numbers are means of duplicate experiments. hCTR1-UTR(F) was used for hCTR1 expression in ctr1.