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Invariant chain constructs. The cassette-containing Ii was cloned into the BamHI/XhoI-digested pcDNAI/amp vector. The cassette is flanked by unique restriction sites SfuI/Eco47III. One-letter codes for the amino acids are given in boldface type. Inserts CLIP, HA, and HSP were generated by annealing the encoding oligonucleotides. Inserts were ligated into the SfuI/Eco47III-digested cassette plasmid, resulting in plasmids pCLIP (wt p31/33 Ii), pHA, and pHSP.
