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Reverse-phase HPLC analysis of HLA-DR1-eluted peptides isolated from transfectant 293-pHA. During a linear gradient of 10–40% of acetonitrile in water containing 0.1% trifluoroacetic acid, 180 fractions were collected. Fraction numbers corresponding to peaks with an absorption of >0.005 absorption unit (AU) at 214 nm are given. The peaks at fractions 28 and 77 are ghost peaks, also present in the reverse-phase HPLC profile of eluates from mock-precipitations (data not shown).
