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. 1997 Jul 8;94(14):7503–7508. doi: 10.1073/pnas.94.14.7503

Figure 1.

Figure 1

Analysis of the expression in insect cells of the recombinant PPV:VLP carrying the 118–132 LCMV CD8+ epitope. (a) Coomassie blue-stained SDS/polyacrylamide gels showing the different steps of PPV:VLP-(LCMV) purification. Lanes: M, molecular mass markers; 1, mock-infected Sf9 cells; 2, crude extract from infected cells; 3, supernatant from lysed cells; 4, pellet from 20% ammonium sulfate precipitation. (b) Immunoblotting analysis of PPV:VLP-(LCMV) with anti-PPV rabbit antiserum (lane 1) and anti-LCMV 118–132 peptide mouse antiserum (lane 2). (c) Electron microscopy analysis of PPV:VLP-(LCMV) particles negatively stained with 2% uranyl acetate.