Figure 2.

In vivo induction of CTL responses by recombinant PPV:VLP carrying the 118–132 LCMV CD8⁺ epitope. BALB/c mice were immunized i.p. on day 0 (A) or on days 0 and 21 (B) with 10 μg of control PPV:VLP or PPV:VLP-(LCMV). Fourteen (A) or 7 (B) days later, spleen cells were in vitro-stimulated for 5 days with the p118–132 peptide and irradiated syngeneic spleen cells. Cytotoxic activity of the effector cells was measured on ⁵¹Cr-labeled P815 target cells pulse-labeled with the p118–132 peptide (•) or incubated with medium alone (▴). Data represent the mean of percent specific lysis from duplicate samples.