Figure 4.

In vivo immunization with PPV:VLP-(LCMV) stimulates a strong virus-specific CTL response. BALB/c mice were injected i.p. on days 0 and 21, with 10 μg of PPV:VLP, 10 μg of PPV:VLP-(LCMV), PBS, or 10⁵ pfu of LCMV. Seven days later, splenocytes were in vitro-stimulated and cytotoxic activity of the effector cells was measured on ⁵¹Cr-labeled J774 target cells infected with LCMV (▪) or incubated with medium (□). Data represent the mean of percent specific lysis from triplicate samples.