Table 1.
Target cells | % specific 51Cr release
|
||||
---|---|---|---|---|---|
Peptide-coated target cells
|
Control target cells
|
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PPV:VLP-(LCMV) | PPV:VLP | PPV:VLP-(LCMV) | PPV:VLP | ||
P815 | (H-2d) | 84.5 | 14.3 | 8.3 | 0 |
EL4 | (H-2b) | 0 | 0 | 0 | 0.5 |
RDM4 | (H-2k) | 0 | 0 | 0 | 0 |
1T22 | (H-2q) | 8.2 | 4.5 | 8.8 | 9.5 |
Mice were immunized i.p. on day 0 with 10 μg of PPV:VLP or 10 μg of PPV:VLP-(LCMV). After 14 days, immune spleen cells were in vitro-stimulated for 5 days with the p118-132 peptide and syngeneic spleen cells. Cytotoxic activity of the effector cells was measured on 51Cr-labeled target cells pulse-labeled with the p118-132 peptide or incubated with medium alone, at a 30:1 effector/target ratio. Data represent mean values of duplicate samples (SD < 10%).