Figure 2.

Comparative growth analyses of wild-type and the fur mutant. Growth curves for strains grown in liquid LB, LB-160uM and LB-240uM iron chelator are shown. Cells grown in mid-logarithmic phase were diluted 1/100 in fresh LB media and growth was monitored every thirty minutes during consecutive five days. Three replicates were used for both strains. The average growth and error bars are shown. The down arrows and up arrows indicate the range of mid-logarithmic phase of MR-1 and the fur mutant, respectively. The doubling time of MR-1 is 1.5 hrs. The doubling time of the fur mutant is 2.5 hrs.