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. 2008 May 14;105(20):7188–7193. doi: 10.1073/pnas.0708044105

Fig. 1.

Fig. 1.

Expression of catalytic mutants of SSAT inhibits α9-dependent migration. (a) 35S-labeled wild-type SSAT (wt), R101A/E152K, R7A, K87A point mutants, and truncation mutants containing C-terminal stop codons at R142 (R142Stop), K161 (K161Stop), or L164 (L164Stop) were produced by in vitro transcription, mixed with α9 cytoplasmic domain fused to GST and glutathione Sepharose 4B, and bound proteins were analyzed by SDS/PAGE and autoradiography. (b) CHO cells expressing α9 integrin subunit alone or with SSAT catalytic mutants migrated for 3 h across filters coated with 5 μg/ml TNfn3RAA. *, P = 2 × 10−9; **, P = 1.5 × 10−15. (c) Migration of CHO cells expressing α9 or α9α5 integrin subunits alone, or with the SSAT catalytic point mutant K87A, analyzed after 3 h as in b. Data in b and c are expressed as mean ± SD. *, P = 0.001.