Figure 3.

Selected pictures illustrating typical changes observed in macrophages (a) and fibroblasts (b) after incubation with 90 mg/L telavancin for 24 or 72 h, respectively. Both cells show markedly enlarged and ill-shaped lysosome-like bodies with a pleiotropic material that is highly osmiophilic (often organized in a distorted concentric fashion). (c) Example of loose material and (d) example of highly osmiophilic material organized in multiple separate bodies. Bars are 1 µm. The dotted lines show examples of profiles selected for morphometric analysis: i, a large profile showing mainly a moderately osmiophilic and pleiomorphic material; ii, a small profile showing mainly osmiophilic material [possibly a polar section of a lysosome with mixed content (as in iii), but cut as shown by the arrow].