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. 2008 Mar 15;61(6):1217–1220. doi: 10.1093/jac/dkn100

Figure 1.

Figure 1

Agarose gel showing the HIV-1 pol amplification signals obtained from DBS specimens stored at 4 or −20°C. Two serial 10-fold dilutions (1/10 and 1/100) of specimens 2 (266 612 RNA copies/mL), 3 (214 330 RNA copies/mL), 4 (191 674 RNA copies/mL) and 10 (49 805 RNA copies/mL) were prepared in Nuclisens elution buffer and were tested in parallel using the ViroSeq assay (1.8 kb) or the in-house RT-nested PCR method (1023 bp). (a) DBS specimens stored at 4°C and amplified using the ViroSeq assay. (b) DBS specimens stored at 4°C and amplified using the in-house method. (c) DBS specimens stored at −20°C and amplified using the in-house method.