Figure 1.
Pseudo-colored images for a comparison of kinetochore protein fluorescence in metaphase budding yeast and G2/M fission yeast cells. Images were obtained under identical imaging and image acquisition conditions. Each of the two spots in a metaphase budding yeast cell contains 16 kinetochores and 16 microtubule attachment sites. In G2/M fission yeast, there are six kinetochores in the single spot with a mean of ∼3 microtubule attachment sites per kinetochore and, therefore, 18 attachment sites in total. It should be noted that microtubule attachments are established only after G2/M transition. The intensity bar is color coded to represent the ratio (indicated by the numbers on the right) with the ScCse4p-GFP intensity in the top panel. Bar, 1 μm.