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. 2008 May 19;181(4):655–666. doi: 10.1083/jcb.200712051

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© 2008 Juhász et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Vps34 does not influence TOR signaling or function. Transgene-expressing cells are marked by coexpression of GFP in A, B, D, and G–J. Loss-of-function mutant clones are marked by lack of GFP in C, E, and F. All images are from fed larvae except G and H, which are from 48-h starved animals. (A and B) Inhibition of TOR signaling by expression of Tor double-stranded RNA (dsRNA; A) or overexpression of Tsc1 and 2 (B) results in a reduction in fat body cell size. (C and D) Fat body cells lacking Vps34 (C) or expressing Vps34^KD (D) are normal in size. (E and F) In the eye imaginal disc, cells mutant for Tor (E; GFP negative) proliferate more slowly than their wild-type “twin spot” control cells (two copies of GFP). Vps34^Δm22 homozygous mutant cells (F) proliferate at a rate similar to that of twin spot controls. (G and H) Overexpression of Rheb activates TOR and increases cell growth both in the absence (G) or presence (H) of coexpressed kinase-defective Vps34. (I and J) Inhibition of TOR signaling by cooverexpression of Tsc1 and 2 reduces cell growth in control (I) and Vps34^Δm22 (J) animals. (K) Ubiquitous hsGAL4-driven overexpression of wild-type Vps34 (lane 2) or kinase-defective Vps34 (lane 3) does not affect TOR-dependent phosphorylation of S6K-Thr398 or Akt-Ser505 relative to control extracts (lane 1). (L) Quantitation of the area of mutant or transgene-expressing cells in A–D relative to wild-type cells from the same tissue. Data represent mean ± SEM of 7–10 animals per genotype. Bar: 10 μm. Genotypes: (A) hsflp; Act>CD2>GAL4 UAS-GFP/UAS-TOR^dsRNA5092R-2, (B) hsflp; Act>CD2>GAL4 UAS-GFP/UAS-Tsc1 UAS-Tsc2, (C) hsflp; FRT42D Vps34^Δm22/UAS-2xeGFP FRT42D fb-GAL4, (D) hsflp; Act>CD2>GAL4 UAS-GFP/UAS-Vps34^KD, (E) hsflp; Tor^ΔP FRT40A/Ubi-GFPnls FRT40A, (F) hsflp; FRT42D Vps34^Δm22/FRT42D Ubi-GFPnls, (G) hsflp; Act>CD2>GAL4 UAS-GFP UAS-Rheb^EP50.084/+, (H) hsflp; Act>CD2>GAL4 UAS-GFP UAS-Rheb^EP50.084/UAS-Vps34^KD, (I) hsflp; Act>CD2>GAL4 UAS-GFP/UAS-Tsc1 UAS-Tsc2, and (J) hsflp; Vps34^Δm22/Vps34^Δm22; Act>CD2>GAL4 UAS-GFP/UAS-Tsc1 UAS-Tsc2.