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. 2008 May 19;181(4):683–695. doi: 10.1083/jcb.200711165

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© 2008 Shen et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Occludin and ZO-1 FRAP are differentially dependent on membrane composition and metabolic energy. (A) FRAP experiments were performed on monolayers of EGFP-occludin– and EGFP–ZO-1–expressing cells incubated at 37 (control), 18, or 14°C, with MBCD, or after ATP depletion. Representative kymographs are shown. (B and C) The t_1/2 and mobile fraction were calculated from FRAP experiments (n ≥ 5 at each temperature). TER was measured in parallel. (D–F) TER and mobile fraction were determined in monolayers incubated with MBCD or after ATP depletion. n ≥ 5 for each treatment. Error bars represent SEM. Bar, 2 μm.