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. 2008 May 19;181(4):697–709. doi: 10.1083/jcb.200801101

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© 2008 Paterson et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Fus2p localization is regulated by the mating response. (A) Mitotically expressed Fus2p-GFP localizes to the nucleus at all stages of mitotic growth. MY9201 containing P_GAL-FUS2-GFP was grown in media containing galactose (top, DIC; bottom, GFP). (B) α-factor treatment causes Fus2p-GFP to exit the nucleus. MY9201 was grown in galactose-containing media and induced with α-factor. As the cell completed mitosis, nuclear Fus2p exited the nucleus and localized to the shmoo tip in pheromone-responding cells (left, DIC at 0 min; remaining panels, GFP). (C) Preexisting nuclear Fus2p-GFP exits the nucleus. MY9201 grown in galactose was transferred to media containing dextrose for the indicated times (10, 20, or 30 min) to shut off expression before treatment with pheromone for 1.5 h. Fus2p-GFP was nuclear in mitotic cells but localized to the shmoo tip in shmoos (top, DIC; bottom, GFP). (D) Fus2p-GFP nuclear exit is dependent on high levels of pheromone. MY9184 was treated with 6 μM α-factor (top) or 0.6 μM α-factor (bottom) and imaged at various times (left, transmitted or DIC at 0 min; right, GFP). (E) Nuclear exit requires the Fus3p MAP-kinase. MY9211 was treated with α-factor for 1.5 h. Transmitted light (first panel), DAPI of the nucleus (second panel), and GFP (remaining panels) are shown. Bars, 1 μm.