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. 2008 May 19;181(4):697–709. doi: 10.1083/jcb.200801101

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© 2008 Paterson et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Rvs161p is required for Fus2p movement and localization. (A) Fus2p-GFP is not transported or localized in an rvs161Δ mutant. MY9214 was induced with pheromone for 1.5 h. (B) Fus2p-GFP localization is dependent on Rvs161p's cell fusion function. MY9231 (rvs161Δ), MY9233 (cell fusion defective allele rvs161-P203Q), and MY9239 (endocytosis-defective allele rvs161-R113K) were treated with α-factor for 1.5 h. Bars, 1 μm. (C) Fus2p interacts with Rvs161p but not Rvs167p. Wild-type cells expressing Rvs167p-HA, Fus2p-FLAG, or both epitope-tagged proteins were treated with α-factor, and proteins were immunoprecipitated with anti-HA or anti-FLAG antibodies. Fus2p-FLAG and Rvs167p-HA could immunoprecipitate Rvs161p but the two tagged proteins did not coimmunoprecipitate with each other. (D) Rvs161p association with Fus2p is dependent on pheromone induction. Fus2p-GFP was expressed from the P_GAL1 promoter (MY9201) by induction with galactose, mock treated, or treated with α-factor for 1.5 h. Protein extracts were prepared and Fus2p-GFP was immunoprecipitated.