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. 2008 May 19;181(4):697–709. doi: 10.1083/jcb.200801101

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© 2008 Paterson et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — Fus2p movement and localization are independent of microtubules. MY9184 expressing Fus2-GFP expressed from its own promoter was treated with α-factor for 1.5 h and either mock treated (A and C) or treated with nocodazole (B and D) for 10 min. Live cell image stacks were acquired (A and B). Arrows indicate directed movement of Fus2p-GFP puncta. Alternatively, cells were fixed and stained with anti-tubulin antibody (C and D) to demonstrate microtubule depolymerization. Bars, 1 μm.