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. 2008 Apr 29;9:13. doi: 10.1186/1471-2091-9-13

Figure 2.

Figure 2

Recombinant MtCS protein expression and purification. (A) Analysis of MtCS expression using E. coli Rosetta(DE3) host cells by SDS-poliacrilamide gel 12%. Lanes: 1 – pellet Rosetta(DE3)+pET23a(+)::aroF; 2 – soluble extract Rosetta(DE3)+pET23a(+)::aroF; 3 – pellet Rosetta(DE3)+pET23a(+) (negative control); 4 – soluble extract Rosetta(DE3)+pET23a(+) (negative control); 5 – protein molecular weight standards, high range (Gibco) (43 kDa; 29 kDa; 18 kDa; 14 kDa) (B) Analysis of purification steps of MtCS by SDS-polyacrylamide gel 12%. Lanes: 1 – soluble extract after dialysis; 2 – soluble extract after Q Sepharose Fast Flow column; 3 – CS after Phenyl-Sepharose column; 4 – CS after MonoQ column; 5 – protein molecular weight standards, high range (Gibco) (200 kDa; 97 kDa; 68 kDa; 43 kDa; 29 kDa; 18 kDa; 14 kDa).