Figure 3.
Elution profile of incubated MtCS and FMNox, and UV-Vis spectrum showing stable binary complex formation. Elution of MtCS and FMNoxincubation in a HiTrapQ HP column upon a linear gradient from 0 to 1 M NaCl (dashed line). The absorbance was monitored at 445 nm (dotted line) and 280 nm (solid line). The first peak corresponds to free FMNox, and the second peak corresponds to MtCS-FMNox complex. Inset: UV-Vis spectrum ranging from 210 nm to 600 nm of the second pool (solid line) and Tris-HCl (dotted line). All characteristic peaks of FMNox can be observed (267 nm, 373 nm, and 445 nm).