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. 2008 Apr 29;9:13. doi: 10.1186/1471-2091-9-13

Figure 7.

Figure 7

Primary and multiple deuterium kinetic isotope effects. Measurements of primary deuterium kinetic isotope effects for MtCS yielded values of 3.5 ± 0.2 and 3.0 ± 0.4 for, respectively, DV and DV/K. Inset: Measurements of primary isotope effect in 90 atom % D2O (multiple) yielded values of 1.3 ± 0.1 and 2.2 ± 0.1 for D2OV[4S-2H]NADH and D2OV/K[4S-2H]NADH, respectively. In both [4S-1H]NADH (●) or [4S-2H]NADH (■) is varied in the reaction mixture. L represents either [4S-1H]NADH or [4S-2H]NADH.